FIG 9.

Both the KSTQT motif in P and the RMTQT motif in L are required for efficient primary transcription. (A) Cells were treated with CHX and 1 h later infected with indicated rRABVs. Cell lysates were prepared after 1 or 24 h, and RABV nucleoprotein N and cellular β-tubulin were detected in Western blots. (B) RNA was prepared after 1 and 24 hpi, and N mRNAs were quantified by qRT-PCR. Shown are data after normalization to actin mRNAs and setting the values of rRABV at 24 hpi in the absence of CHX to 1. Standard deviations (n = 3 independent infections) are shown by error bars. (C) For better comparison at 24 hpi, values for rRABV in the absence (− CHX) and presence (+ CHX) of cycloheximide were set to 1. Standard deviations (n = 3 independent infections) are shown by error bars.