FIG 3.

ADCML of HIV-1 virions of primary isolates by nAbs and non-nAbs. (A) HIV-1 virions of the six primary isolates were incubated with BRIC229 (20 μg/ml) to block hCD59. Nonspecific IgG was used at 20 μg/ml as a negative control of BRIC229. Virions were treated with individual nAbs or non-nAbs as indicated (5 μg/ml), followed by exposure to 10% of the pooled sera from three healthy blood donors as a source of complement. In parallel experiments, heat-inactivated sera from the same healthy blood donors were used as a negative control of complement activity. Virolysis was analyzed by ELISA titration of released HIV-1 Gag proteins. In each experiment, treatments with culture medium alone or with Triton X-100 were used as blank and 100% virolysis, respectively. Means ± SD from three experiments performed in duplicate are shown. (B) Pooled virolysis data from HIV-1 virions of all six primary isolates. Statistical significance (**, P < 0.01 versus medium or IgG treatment group) is indicated by asterisks. ns, not significant.