FIG 5.

Anti-Env nAbs and non-nAbs triggered ADCML of latently HIV-1-infected cells upon provirus activation. Specific lysis of provirus-activated ACH-2 cells by anti-Env nAbs and non-nAbs. The calcein release assay was used to quantitate ADCML of latently HIV-1-infected cells by anti-HIV-1 nAbs and non-nAbs. ACH-2 or A3.01 cells treated or untreated with PMA/ionomycycin or DMSO. Cells were preincubated with BRIC229 (20 μg/ml) to block hCD59 function. An irrelevant IgG control Ab (20 μg/ml) was used in parallel as a negative control of hCD59 blockage. After preincubation, nAbs or non-nAbs were added, followed by the exposure to either complement-competent or heat-inactivated sera from three healthy blood donors. Triton X-100 was used for determining the total cytolysis. IgG + C, irrelevant Ab IgG plus complement; BRIC229 + C, BRIC229 Ab plus complement; BRIC229 + iC, BRIC229 Ab plus inactivated complement. Statistical significance (**, P < 0.01 versus IgG plus complement or BRIC229 plus inactivated complement) is indicated by asterisks. ns, not significant.