FIG 4.

BSE and CWD prions passed into felines demonstrate either (i) maintenance of original species characteristics (BSE) or (ii) adaptation to the new species (CWD). (A) Points indicate the average rate of amyloid conversion for white-tailed deer rPrP (black line) or feline rPrP (green line) upon seeding with an fCWD seed. (B) Points indicate the rate of amyloid conversion for bovine rPrP (blue line) or feline rPrP (green line) upon addition of an FSE seed. The rate of spontaneous amyloid conversion is designated by the dotted gray line, and error bars represent SEM. Significant differences between substrates were tested by the MWU test (*, P < 0.05) or the WSR test (#, P < 0.05). (C, D) Each line represents the average ThT trace for 6 replicates of 3 dilutions of each seed-substrate combination, and the gray line indicates the threshold for determination of the lag phase.