FIG 5.

CWD is capable of efficiently seeding the conversion of human rPrP^C. (A) Points represent the average rate of conversion of full-length human M129 rPrP by sporadic CJD (black line), CWD (red line), fCWD (pink line), BSE (dark blue line), and FSE (light blue line). Error bars indicate SEM. The rate of spontaneous amyloid conversion is indicated by the gray line. (B) Western blot of BSE and CWD. PK-digested lanes indicate the presence of more PrP^Res in the BSE sample than in the CWD sample. Densitometry indicates that BSE has more PrP^Res/microliter relative to total undigested PrP/microliter than does CWD. (C) Points indicate the average rate of amyloid conversion for multiple field isolates of CWD in full-length human M129 rPrP^C. The gray line indicates the rate of spontaneous amyloid conversion. (D) Points indicate the average rate of amyloid conversion for multiple field isolates of CWD in full-length white-tailed deer rPrP^C. The gray line indicates the rate of spontaneous amyloid conversion.