FIG 7.

Lack of induction of dsRNA reactivity with CXH and CXH plus TNF-α, which results in apoptosis, in uninfected M1-D macrophages. (A) Uninfected cells were incubated with 500 μM CXH for 6 h before staining with MAb 9D5 and showed no dsRNA reactivity. (B) TMEV-infected cells showing cytoplasmic dsRNA staining as a positive control. (C) DAPI-stained fragmented and blebbing nuclei, indicative of apoptosis, in uninfected cells. (D) Immunoblot analysis showing PARP and caspase-3 (Casp-3) cleavages, indicative of apoptosis, when cells were incubated with CHX at concentrations of ≥50 μM and slightly more robust cleavages when cells were incubated with both CHX at a concentration of 50 or 100 μM and TNF-α at a concentration of 5 or 10 μg.