FIG 4.

Murine Ifit1 does not restrict infection by OROV, LACV, and maEBOV. (A and B) Kinetics of OROV (A) and LACV (B) replication in WT and Ifit1^−/− MEFs, with or without 18 h of IFN-β (20 U/ml) stimulation prior to infection at an MOI of 0.01. Supernatants were harvested at the indicated times for titration by a focus-forming assay. Two independent experiments were performed, each with seven technical replicates. (C) Kinetics of maEBOV replication in WT and Ifit1^−/− Mϕ, with or without 18 h of IFN-β (100 U/ml) stimulation before infection at an MOI of 0.01. Supernatants were harvested at the indicated times for titration by a TCID₅₀ assay. A dotted line represents the limit of detection of the assay. The data represent the means ± SD for two independent experiments performed in triplicate. No statistical differences were observed as judged by the Mann-Whitney test.