FIG 7.

Murine Ifit1 does not affect virus infection or pathogenesis in vivo after infection with OROV or LACV. (A and B) Morbidity and survival analyses of 8-week-old WT (n = 15) and Ifit1^−/− (n = 13) mice after inoculation with 10⁵ FFU of LACV by subcutaneous injection in the footpad. Data were pooled from three independent experiments. (C) Viral burdens in the liver, spleen, brain, and serum on day 8 after LACV infection of WT and Ifit1^−/− mice were measured by quantitative RT-PCR. A scatterplot of the data is shown, with each individual point representing a single animal (n = 10 to 12). The dashed line represents the limit of sensitivity of the assay. Statistical significance was judged by the Mann-Whitney test. (D and E) Morbidity and survival analyses of 6-week-old WT (n = 23) and Ifit1^−/− (n = 10) mice after inoculation with 10⁶ FFU of OROV by subcutaneous injection in the footpad. Data were pooled from three independent experiments. (F) Viral burdens in the liver, spleen, brain, and serum on day 4 after OROV infection of WT and Ifit1^−/− mice were measured by quantitative RT-PCR. Viral burdens are recorded as per-gram values for organs (left axis) and per-milliliter values for serum (right axis). A scatterplot of the data is shown, with each individual point representing a single animal (n = 10 to 13). The dashed line represents the limit of sensitivity of the assay. No statistical differences were observed as judged by the Mann-Whitney test.