TABLE 1.

Difference in dissociation constants of murine Ifit1 for RNA oligonucleotides representing influenza virus genomic RNA^a

5′-End modification	Fold difference in KD of murine Ifit1 for IAV gene segment
	PB2	PB1	NS
cap 0 → cap 1	5	16	7
cap 0 → 5′-ppp	11	17	6

^aA filter-binding assay was used to measure the binding affinity of murine Ifit1 for IAV 5′-UTR cap 0, cap 1, and 5′-ppp. The K_D (micromolar) was calculated by using the nonlinear Hill equation, using Origin. The data presented are fold differences in K_D values between cap 0 and cap 1 or cap 0 and 5′-ppp RNA, calculated from two independently repeated assays.