. 2015 Aug 19;89(18):9465–9476. doi: 10.1128/JVI.00996-15

TABLE 2.

Cytokine and chemokine levels in lungs of WT and Ifit1^−/− mice after influenza virus infection^a

Cytokine	Concn (pg/ml) (mean ± SD) in mouse lung
	Day 3		Day 6		Day 9
	WT	Ifit1^−/−	WT	Ifit1^−/−	WT	Ifit1^−/−
IL-1α	31 ± 3	33 ± 5	52 ± 15	45 ± 3	16 ± 3	16 ± 3
IL-1β	319 ± 13	326 ± 30	787 ± 76	852 ± 197	214 ± 25	227 ± 26
IL-2	23 ± 11	24 ± 11	35 ± 12	35 ± 12	29 ± 13	33 ± 13
IL-3	4 ± 10	5 ± 10	14 ± 12	13 ± 11	8 ± 12	8 ± 10
IL-4	4 ± 10	5 ± 11	36 ± 3	37 ± 3	23 ± 5	22 ± 6
IL-5	65 ± 9	67 ± 18	214 ± 33	397 ± 73	21 ± 9	21 ± 6
IL-6	297 ± 32	231 ± 37	413 ± 40	416 ± 178	127 ± 7	57 ± 4
IL-10	21 ± 1	23 ± 2	43 ± 7	67 ± 38	85 ± 13	82 ± 20
IL-12(p40)	253 ± 18	270 ± 22	49 ± 13	77 ± 67	38 ± 8	22 ± 3
IL-12(p70)	39 ± 4	32 ± 2	821 ± 76	772 ± 51	235 ± 49	259 ± 45
IL-13	ND	ND	79 ± 32	92 ± 22	90 ± 28	74 ± 26
IL-17	ND	ND	8 ± 21	12 ± 11	37 ± 34	33 ± 34
Eotaxin	613 ± 76	455 ± 79	489 ± 58	657 ± 50	6 ± 11	5 ± 14
G-CSF	294 ± 41	315 ± 67	743 ± 70	821 ± 89	299 ± 63	114 ± 23
GM-CSF	63 ± 31	64 ± 4	52 ± 41	55 ± 21	38 ± 53	33 ± 21
IFN-γ	9 ± 21	8 ± 11	40 ± 11	93 ± 11	ND	ND
KC	3,303 ± 242	2,985 ± 588	1,635 ± 155	1,383 ± 168	107 ± 22	151 ± 30
CCL2	1,272 ± 178	1,018 ± 122	1,357 ± 464	2,216 ± 243	698 ± 111	474 ± 114
CCL3	182 ± 29	145 ± 19	767 ± 42	931 ± 158	111 ± 28	87 ± 25
CCL4	134 ± 18	128 ± 24	121 ± 12	267 ± 39	58 ± 11	53 ± 8
CCL5	395 ± 57	284 ± 55	513 ± 36	515 ± 53	120 ± 20	152 ± 44
TNF-α	296 ± 40	254 ± 46	323 ± 23	332 ± 23	239 ± 42	282 ± 44

WT and Ifit1^−/− mice (n = 9) were infected with 10⁴ EID₅₀ of IAV-Cal, and 3, 6, or 9 days after infection, lungs were harvested, homogenized, and analyzed by the Bio-Plex Pro 23-plex mouse cytokine and chemokine assay. Data were pooled from three independent experiments. ND, levels were below the threshold of detection. No significant differences (P > 0.08) were observed between WT and Ifit1^−/− mice for any of the cytokines or chemokines for any day tested.