FIG 7.

Calcium signaling induced by monomeric gp120 is inhibited by NF279. Changes in cytosolic calcium levels in TZM-bl cells were measured as described in Materials and Methods and the legend of Fig. 6. Recombinant gp120 (130 nM) (arrows) was applied to cells in the absence (A) or in the presence (B) of 30 μM NF279. Graphs in panels A and B show changes in the Fluo4 signals from individual cells (n = 28 and n = 30, respectively) after normalization to the signal elicited by 2.5 μM A23187. Fluorescence traces are from a representative experiment done in triplicate. Image panels below the graphs show snapshots of cells at the indicated time points (bar, 50 μm). The last image in each panel was acquired after the addition of A23187.