Table 3.

Analysis of circulating TGF-β1–regulated miRNA levels in rapid progressors, nonprogressors, and normoalbuminuric control subjects

miRNA	Relative miRNA levels*			Kruskal-Wallis ANOVA P	Mann-Whitney U test P (RP vs. NP)
	RP	NP	Normoalbuminuric control subjects
let-7b-5p	8.0 × 10−4 ± 9.8 × 10−4	3.4 × 10–4 ± 4.2 × 10−4	1.9 × 10−4 ± 2.6 × 10−4	0.0003	0.01
let-7c-5p	1.7 ± 1.6	3.1 ± 2.1	3.7 ± 2.7	<0.0001	0.0002
miR-21-5p	9.9 × 10−4 ± 1.4 × 10−3	2.2 × 10–4 ± 3.8 × 10−4	5.6 × 10−5 ± 6.0 × 10−5	<0.0001	0.006†
miR-29a-3p	2.17 ± 3.05	3.5 ± 2.4	4.2 ± 2.7	<0.0001	0.0007
miR-29c-3p	1.1 × 10−3 ± 1.3 x10−3	1.3 × 10−3 ± 1.8 × 10−3	6.2 × 10–4 ± 1.2 × 10−3	0.0003	0.68‡

Data are means ± SD. NP, nonprogressors; RP, rapid progressors.

*The relative level of each miRNA was calculated according to the equation 2^−ΔCq, where ΔCq = average Cq_{TGF-β1–regulated miRNA} – average Cq_c.el-miR-39.

†miR-21-5p was significantly upregulated in both rapid progressors (P < 0.0001) and nonprogressors (P = 0.02) compared with normoalbuminuric control subjects.

‡miR-29c-3p was significantly increased in both rapid progressors (P = 0.0009) and nonprogressors (P = 0.0003) compared with normoalbuminuric control subjects.