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. 2015 May 28;64(9):3203–3217. doi: 10.2337/db14-1938

Figure 1.

Figure 1

GPR43 is expressed in pancreatic β-cells and is increased by HFD. A: GPR43 mRNA expression in murine tissues. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; IP Mac, intraperitoneal macrophage. Data are reported as the mean ± SEM, n = 4. B: GPR43 expression in murine islets and eWAT isolated from NC or HFD mice. Data are reported as the mean ± SEM, n = 4. *P < 0.05, NC vs. HFD. C: GPR43 expression in murine and human islets. Data are reported as the mean ± SEM, n = 4. D: Immunofluorescence analysis of GPR43 expression in murine islets. Anti-GPR43 is shown in red, and anti-insulin is shown in green. E: Serum acetate levels in WT and KO mice fed an NC diet and a 14-week HFD, and fasted mice. Data are reported as the mean ± SEM, n = 6 per group. *P < 0.05. F–H: Real-time PCR quantification of fecal bacterial phyla abundance, as a percentage of total bacteria, in WT and KO mice fed an NC diet and a 14-week HFD. F: Abundance of Firmicutes and Bacteroidetes. G: Abundance of Actinobacteria. H: Abundance of Gammaproteobacteria. I: Relative fecal mRNA expression of BCoAT and FTHFS in WT and KO mice fed an NC diet and an HFD. NC diet–fed mice, n = 8 per group. HFD-fed WT mice, n = 9; HFD-fed KO mice, n = 6. Data are reported as the mean ± SEM. *P < 0.05.