Figure 4.

GPR43-stimulated insulin secretion is dependent on PLC. A: Min6 GSIS in the presence of PLC inhibitor. Black bars, control; white bars, PLC inhibitor. B: Min6 GSIS in the presence of PTX. Black bars, control; white bars, PTX. C: IP₁ generation in Min6 cells treated with 1 mmol/L acetate or 1 μmol/L PA. D: Forskolin-stimulated cAMP production in Min6 cells. Data are reported as the mean ± SEM of three independent experiments. E: Ins-1 GSIS in the presence of PLC inhibitor. Black bars, control; white bars, PLC inhibitor. F: Ins-1 GSIS in the presence of PTX. Black bars, control; white bars, PTX. G: IP₁ generation in Ins-1 cells treated with 1 mmol/L acetate or 1 μmol/L PA. H: Forskolin-stimulated cAMP production in Ins-1 cells. Data are reported as the mean ± SEM of three independent experiments. I: IP₁ production isolated islets from mice fed an NC diet, a 5-week HFD, and a 14-week HFD. J: Forskolin-stimulated cAMP production in isolated islets from mice fed an NC diet, a 5-week HFD, and a 14-week HFD. Data are reported as the mean ± SEM, n = 4–5 per group. K: IP₁ production in human islets. L: Forskolin-stimulated cAMP production in human islets. Data are reported as the mean ± SEM, n = 3. M: Cytosolic calcium in Min6 cells treated with 1 μmol/L PA. Dashed vertical line indicates the addition of DMSO (control) or PA. Data are reported as the mean ± SEM of three independent experiments. *P < 0.05. A, acetate; Ctr, control.