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Schema of islet cell subset separation and frequencies of donor islet cell subsets. A: Islets are composed of a heterogeneous mixture of α-cells (GCG+), β-cells (INS+), and δ-cells (SST+). Islets are dissociated into a single-cell suspension (B), fixed and permeabilized to stain for intracellular hormone content (C), and sorted using a FACS (D) into distinct populations. E: Composition of endocrine islet populations of α- and β-cells differs from donor to donor.
