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. 2015 May 6;64(9):3111–3120. doi: 10.2337/db14-1627

Figure 5.

Figure 5

SIRT1 inhibits LPL expression by suppressing PPARγ expression in trophoblasts. To verify the relationship between SIRT1 and PPARγ in regulating LPL expression, Sirt1−/− MEFs (A) and JEG-3 trophoblasts (B) were treated with PPARγ agonist ROSI (10 μmol/L) for 13 h. ROSI treatment robustly increased LPL mRNA in Sirt1−/− MEFs (n = 6) (A) but showed no effect on SIRT1 protein expression in trophoblasts (n = 6) (B). By using viral vector–mediated small interfering RNA (siRNA) overexpression, Sirt1 was significantly knocked down in JEG-3 trophoblasts (C). However, increased LPL expression due to Sirt1 knockdown was abolished in PPARγ inhibitor GW9662-treated cells (3 μmol/L, 13 h) (n = 6) (C). *P < 0.05 vs. control. Con, control.