Table 1. Description of characterized NAT loci.
| Species (strain) | Taxon mnemonic1 | Taxon ID1 | Gene symbol | ORF (bp)2 | Protein (aa)2 | Exon span | Number of introns | Predicted locus tag3 | Nucleotide ID4 | |
|---|---|---|---|---|---|---|---|---|---|---|
| F. verticillioides (FGSC 7600) | GIBM7 | 334819 | ΝΑΤ1 | 1038 | 345 | 1 (1–1038) | 0 | FVEG_12636 | EU552489, FN687904 | |
| ΝΑΤ2 | 957 | 318 | 1 (1–957) | 0 | FVEG_03961 | FN687889, FN687905 | ||||
| ΝΑΤ3 | 978 | 325 | 1 (1–978) | 0 | FVEG_12062 | FN687890, FN687906 | ||||
| NAT45 | Transcribed pseudogene | 1 (1–298) 2 (461–1033) | 1 | FVEG_07425 | FN687891, LN829129 | |||||
| F. graminearum (PH-1) | GIBZE | 229533 | ΝΑΤ1 | 1032 | 343 | 1 (1–1032) | 0 | FGSG_00080 | FN687882, FN687897 | |
| ΝΑΤ2 | 957 | 318 | 1 (1–371) 2 (430–1015) | 1 | FGSG_09400 | FN687883, FN687898 | ||||
| ΝΑΤ3 | 960 | 319 | 1 (1–960) | 0 | FGSG_07888 | FN687884, FN687899 | ||||
| F. oxysporum f.sp. lycopersici (FOL 4287) | FUSO4 | 426428 | ΝΑΤ1 | 1053 | 350 | 1 (1–1053) | 0 | FOXG_15318 | FN687885, FN687900 | |
| ΝΑΤ2 | 957 | 318 | 1 (1–371) 2 (423–1008) | 1 | FOXG_06095 | FN687886, FN687901 | ||||
| ΝΑΤ3 | 999 | 332 | 1 (1–999) | 0 | FOXG_03795 | FN687887, FN687902 | ||||
| ΝΑΤ4 | 963 | 320 | 1 (1–365) 2 (548–1145) | 1 | FOXG_04301 | FN687888, FN687903 | ||||
| A. flavus (NRRL 3357) | ASPFN | 332952 | NAT15 | Elusive | Elusive | Elusive | Elusive | AFL2G_05055 | − | |
| ΝΑΤ2 | 981 | 326 | 1 (1–426) 2 (482–528) 3 (588–1095) | 2 | AFL2G_01915 | FN687893, FN687907 | ||||
| ΝΑΤ3 | 957 | 318 | 1 (1–395) 2 (449–1010) | 1 | AFL2G_11316 | FN687894, FN687908 | ||||
| ΝΑΤ45 | Transcribed pseudogene | 1 (1–91) 2 (143–692) | 1 | AFL2G_03311 | FN687895, FN687909 | |||||
| A. nidulans (FGSC A4) | EMENI | 227321 | ΝΑΤ1 | 960 | 319 | 1 (1–407) 2 (463–510) 3 (568–1072) | 2 | ANID_10723 | FN687881, FN687896 | |
1The taxon mnemonics and ID numbers are from the UniProt Taxonomy database (http://www.uniprot.org/taxonomy/). They correspond to sequenced strains of Fusarium verticillioides (teleomorph Gibberella moniliformis), Fusarium graminearum (teleomorph Gibberella zeae), Fusarium oxysporum f.sp. lycopersici, Aspergillus flavus and Aspergillus nidulans (teleomorph Emericella nidulans). According to current consensus nomenclature guidelines (Supplementary Methods and http://nat.mbg.duth.gr/), taxon mnemonics are attached to the symbols of NAT genes to identify their specific organism of origin.
2The sequences of open reading frames (ORF) in base pairs (bp), as well as of deduced proteins in amino acids (aa), were determined via alignment of amplification products generated from genomic DNA and cDNA.
3The locus tags represent annotations by the Broad Institute (http://www.broadinstitute.org/science/projects/fungal-genome-initiative/gene-finding-methods). F. verticillioides FVEG_07425 and A. flavus AFL2G_03311 tag genomic loci with sequences overlapping, but not coinciding, with the NAT sequences characterized experimentally in the present study.
4Two Nucleotide IDs were assigned to each fungal NAT, the first for the genomic and the second for the transcribed sequence of each locus.
5Annotation remains elusive for the NAT1 locus of A. flavus, as specific amplification from cDNA of the fungus has not been possible. The NAT4 loci of F. verticillioides and A. flavus appeared as transcribing pseudogenes with hypothetical ORFs that are disrupted by nonsense mutations.