Table 1.
List of bacterial strains used.
| Species and strain | Genotypic and phenotypic characteristics4 | Source |
|---|---|---|
| Pseudomonas aeruginosa PAO1 | Lee et al. (2014) | |
| PAO1Δalg | Isogenic alg8 deletion mutant | Ghafoor et al. (2011) |
| PAO1Δpel | Isogenic pelF deletion mutant | Ghafoor et al. (2011) |
| PAO1Δpsl | Isogenic pslA deletion mutant | Ghafoor et al. (2011) |
| PDO300ΔmucA | Mutation in the mucA22 allele | Mathee et al. (1999) |
| PAO1-eYFP | Carries the gene encoding eYFP in the intergenic region between coding region of glmS and its downstream gene; GmR | Lee et al. (2014) |
| PAO1Δalg-eYFP | This project | |
| PAO1Δpel-eYFP | This project | |
| PAO1Δpsl-eYFP | This project | |
| PDO300ΔmucA-eYFP | This project | |
| 1P. protegens Pf-5 | Lee et al. (2014) | |
| Pf-5-eCFP | Carries the gene encoding eCFP in the intergenic region between coding region of glmS and its downstream gene; GmR | Lee et al. (2014) |
| 2Klebsiella pneumoniae KP-1 | Lee et al. (2014) | |
| KP-1 -DsRed | Carries the gene encoding DsRedExpress in the intergenic region between coding region of glmS and its downstream gene; GmR | Lee et al. (2014) |
| Escherichia coli | ||
| JM109 | endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ Δ(lac-proAB) e14- [F′ traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+) | Yanisch-Perron et al. (1985) |
| HPS1 | F- Δ(lab-proAB) endA1 gyrA96 hsdR17 supE44 relA1 recA1 thi rifR zzx::mini-Tn5Lac4 | Choi et al. (2005) |
| CC118 aaaaaapir | Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recAl aaaaaa pir | Choi et al. (2005) |
| DH5α aaaaaapir | F-,Φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK-, mK+) phoA supE44 thi-1 aaaaaa pir | Choi et al. (2005) |
| S17-1 aaaaaapir | hsdR recA pro RP4-2 (Tc::Mu; Km::Tn7; aaaaaa pir) | Miller and Mekalanos (1988) |
| HB101 | F-, hsdS20 (rb-, mb-), supE44, ara14, galK2, lacY1, proA2, rpsL20 (StrR), xyl-5, mtl-1, l-, recA13, mcrA-, mcrB- |
Lambertsen et al. (2004) |
1Pseudomonas fluorescens Pf-5 has recently been renamed as P. protegens Pf-5 (Ramette et al., 2011; Lim et al., 2013)
2Klebsiella pneumoniae is an environmental isolate, has been sequenced and the sequence deposited under the accession number- AVNZ01000000, AVNZ00000000 (Lee et al., 2013).
GmR, Gentamicin resistance; StrR, Streptomycin resistance.