Table 2.
Plasmid | Relevant characteristic3 | Source |
---|---|---|
pTNS1 | Helper plasmid, providing the Tn7 transposition function. ApR, R6K ori, ori T | Choi et al. (2005) |
pTNS2 | Helper plasmid, providing the Tn7 transposition function. ApR, R6K ori, ori T | AY8848331,2 |
pTNS2-ColE1 | Helper plasmid, providing the Tn7 transposition function. ApR, ColE1 ori, ori T | Lee et al. (2014) |
pUC18T- mini-Tn7T-Gm-eYFP/HPS1 | pUC18 –based delivery plasmid for mini-Tn7-Gm-eYFP. ApR, GmR, ColE1 ori, oriT | DQ4938791,2 |
pUC18TR6K- mini-Tn7T-Gm-eYFP | pUC18 –based delivery plasmid for mini-Tn7-Gm-eYFP. ApR, GmR, R6K ori, oriT | Lee et al. (2014) |
pRK600 | Mobilizing plasmid, providing the mobilization ability during conjugation. ApR, CmR, R6K ori | Laboratory stock |
pUC18TR6K-mini-Tn7T | pUC18 –based vector plasmid for construction of R6K replicon-based delivery plasmids in this project. ApR, R6K ori, oriT | AY7129532 |
1Plasmids were generously provided by Herbert P. Schweizer (Choi et al., 2005).
2National Center for Biotechnology Information (NCBI) accession number.
3ApR, Ampicillin resistance; CmR, Chloramphenicol resistance; GmR, Gentamicin resistance.