Table 1. Bioactivity of sulfakinin and sulfakinin-related peptides on the two Tribolium castaneum sulfakinin receptors (TcSKR1 and TcSKR2) in Sf9 cell-based reporter system.
| Peptide | Sequence | relative activity (% nsSK)# |
|
|---|---|---|---|
| TcSKR1 | TcSKR2 | ||
| nsSK | FDDYGHMRF-NH2 | 100.0 ± 0.0* | 100.0 ± 0.0* |
| 2003 | FDDYGHMRA-NH2 | −2.0 ± 3.4 | −3.5 ± 5.9 |
| 2004 | FDDYGHMAF-NH2 | 58.0 ± 22.0 | 18.7 ± 10.2 |
| 2005 | FDDYGHARF-NH2 | −0.8 ± 4.4 | 11.9 ± 2.7 |
| 2006 | FDDYGAMRF-NH2 | 0.5 ± 0.7 | 4.0 ± 9.9 |
| 2007 | FDDYAHMRF-NH2 | 55.0 ± 7.1 | 8.5 ± 5.3 |
| 2008 | FDDAGHMRF-NH2 | 5.3 ± 1.5 | 21.5 ± 10.7 |
| 2009 | DDYGHMRF-NH2 | 7.8 ± 11.8 | 1.2 ± 13.7 |
| 2010 | DYGHMRF-NH2 | 28.1 ± 15.6 | 8.6 ± 9.1 |
| 2011 | YGHMRF-NH2 | 2.8 ± 2.5 | 11.7 ± 13.5 |
| 2053 | GHMRF-NH2 | 0.6 ± 0.1 | −4.8 ± 0.1 |
| 2052 | HMRF-NH2 | 6.8 ± 0.2 | 1.8 ± 0.1 |
| 2051 | MRF-NH2 | 4.4 ± 12.0 | 8.3 ± 6.6 |
| 1835 | SDDY(SO3H)GHMRF-NH2 | 76.5 ± 7.6* | 645.7 ± 126.6* |
| 1591-1 | EADY(SO3H)GHNleRF-NH2 | 82.6 ± 2.0* | 113.7 ± 60.5* |
| 1658 | DDY(SO3H)GHMRF-NH2 | 64.8 ± 8.4* | 516.8 ± 50.6* |
#Relative bioactivity of a peptide calculated at concentration of 10 μM and related to the activity of nonsulfated SK (nsSK) peptide. Data are represented as mean ± SEM from at least two biological replicates.
*A peptide is active on the sulfakinin receptor (SKR).