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. 2015 Aug 20;5:13283. doi: 10.1038/srep13283

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Copyright © 2015, Macmillan Publishers Limited

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(A) Schematic representation of the recombination between YFP and CFP genes in FRET biosensors in the process of lentiviral or retroviral gene transfer. Two copackaged genomic RNAs encoding FRET biosensors are included in a virus particle. After infection, cells express only YFP or CFP. (B) FRET biosensors with different YFP variants. A PKA FRET biosensor, AKAR3EV, is composed of YPet (YFP), a FHA1 domain, linker, PKA substrate, nTurquoise-GL (CFP), and a nuclear export sequence (NES). In this study, YPet is replaced with h100YPet, H75-e25YPet, h50-e50YPet, h25-e75YPet, e100YPet, e75-h25YPet, e50-h50YPet, and e25-h75YPet.