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. 2015 Aug 20;5:13283. doi: 10.1038/srep13283

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(A) The recombination of FRET biosensors with h25-e75YPet generates CFP, which includes the critical amino acid substitution of Y66W from GFP. (B) The recombination of FRET biosensors with e75-h25YPet generates GFP or YFP, which includes the critical amino acid substitution of T203Y from GFP.