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. 2015 Aug 20;5:13283. doi: 10.1038/srep13283

Figure 5. Effect of a short spacer between YFP and CFP on recombination.

Figure 5

(A) Schematic representation of a FRET biosensor with YPet, GGSGG linker, nTurquoise-GL, and NES. (B,C) HeLa cells were infected with lentivirus encoding FRET biosensor with a short spacer, 15 bases (B) and full spacer, 812 bases (C). At least 4 days after infection, 300 cells were imaged with an epi-fluorescence microscope, and represented as in Fig. 3. Note that panel C is the same graph as in Supplementary Fig. 2A. Orange and cyan arrowheads indicate the T203Y and Y66W positions, respectively.