Table 1.

Nuclear patterns defined by reactivity with distinct nuclear compartments in interphase cells and staining of mitotic cells.

	Nucleoplasm	Nucleoli	Metaphase chromosomal plate	Metaphase cytoplasm	Mitotic apparatus	Interphase cytoplasm
Homogeneous (AC-1)	Homogeneous	Negative/positive	Homogeneous	Negative	Negative	Negative
Speckled
Dense fine speckled (AC-2)	Heterogeneous fine speckles	Negative	Heterogeneous fine speckles	Negative	Negative	Negative
Fine speckled (AC-4)	Uniform fine speckles	Negative/positive	Negative	Diffuse fine speckles	Negative	Negative
Large/coarse speckled (AC-5)	Variably sized large speckles	Negative	Negative	Diffuse fine speckles	Negative	Negative
Discrete nuclear dots
Centromere (AC-3)	~30–50 dots	Negative	~30–50 aligned dots	Negative	Negative	Negative
Multiple nuclear dots (AC-6)	~10 dots	Negative	Rarely occasional dots	Negative/positive	Negative	Negative
Few nuclear dots (AC-7)	1–6 dots	Negative	Rarely occasional dots	Negative	Negative	Negative
Nucleolar
Homogeneous (AC-8)	Negative	Homogeneous	Negative	Diffuse homogeneous	Negative	Negative
Clumpy (AC-9)	Negative	Large granular	Positive (peri-chromosomal)	Negative/positive	Negative	Negative
Punctate (AC-10)	Negative	Fine speckled	1–5 bright pairs of spots (NOR)	Negative/positive	Negative	Negative
Nuclear envelope
Smooth NE (AC-11)	Linear staining of NE	Negative	Negative	Diffuse	Negative	Negative
Punctate NE (AC-12)	Granular staining of NE	Negative	Negative	Homogeneous/dense speckled	Negative	Negative
Pleomorphic
PCNA-like (AC-13)	Variably sized speckles in S-phase cells (~30%)	Positive in late S-phase	Negative	Negative	Negative	Negative
CENP-F-like (AC-14)	Fine granular in G2-phase	Negative	~30–50 aligned dots	Diffuse	Midbody	Negative

Amber background are recommended as competent-level reporting, whereas all others (Olive green) are considered for expert-level reporting.