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. 2015 Aug 6;5:12937. doi: 10.1038/srep12937

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cDNAs of PB memory-mECs (corresponding to C1.1 for RA1 and C2.1 for RA2 as shown in Fig. 1) and memory-NECs obtained by the single-cell analysis were mixed (4 pooled samples in total), and qPCR was performed. ACTB was used as an internal control gene. *p < 0.05, **p < 0.005.