Platform | Range of effective density (cells/cm2) | Range of cell number to plate | Suggested initial media volume (µl) | Suggested media volume after day 1 (µl) |
6-well plate | 10,500 - 36,500 | 99,750 - 346,750 | 1,000 | 2,000 |
24-well plate | 15,600 - 46,800 | 29,640 - 88,920 | 500 | 1,000 |
96-well plate | 103,500 - 260,000 | 33,120 - 83,200 | 100 | 200 |