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. 2015 May 14;(99):52823. doi: 10.3791/52823
Platform Range of effective density (cells/cm2) Range of cell number to plate Suggested initial media volume (µl) Suggested media volume after day 1 (µl)
6-well plate 10,500 - 36,500 99,750 - 346,750 1,000 2,000
24-well plate 15,600 - 46,800 29,640 - 88,920 500 1,000
96-well plate 103,500 - 260,000 33,120 - 83,200 100 200