Table 3.
Immunohistochemical panel and results
| Antigen/antibody/clone | Company | Dilution | Result |
|---|---|---|---|
| S-100 protein | Dako | 1:2,000 | 12/12 (100 %); strong, diffuse, nuclear and cytoplasmic positive |
| Glial fibrillary acidic protein (GFAP)(6F2) | Dako | 1:200 | 7/12 (58 %); weak to strong, focal cytoplasmic positive |
| SOX10 (EP268) | Epitomics | 1:250 | 7/12 (58 %); strong, focal, nuclear |
| NFP (2F11) | Dako | Neat | 9/12 (75 %); weak to strong, diffuse, cytoplasmic |
| Epithelial membrane antigen (EMA)(E29) | Ventana medical systems | Neat | 1/12 (8 %); strong, focal, cytoplasmic |
| CD34 (QBEnd/10) | Ventana medical systems | Neat | 11/12 (92 %); strong, diffuse, cytoplasmic (adjacent to neural cells) |
| bcl-2 | Dako | 1:40 | 11/12 (92 %); weak to strong, diffuse, cytoplasmic positive |
| INI-1 (BAF47) | Sigma-Aldrich | Neat | 12/12 (100 %); strong, diffuse, nuclear (intact) positive |
| p63 (7jul) | Leica microsystems | 1:40 | 1/12 (8 %); strong, focal, nuclear positive |
| Cytokeratin-pan (AE1/AE3:M3515) | Dako | 1:40 | 0/12 (0 %) |
| Smooth muscle actin (66.4.C2) | Leica microsystems, buffalo grove, IL | 1:200 | 0/12 (0 %) |
| CD117 (C-Kit) | Dako | 1:400 | 0/12 (0 %); positive in mast cells |
| β-Catenin | Cell signal | 1:1,800 | 10/12 (83 %); weak to strong, diffuse, cytoplasmic positive (no nuclear stain) |
| Calretinin | Zymed | Neat | 4/12 (33 %); strong, focal, nuclear (in addition to mast cells) positive |