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Mechanical stretch activates mTORC1 in HL-1 cells. Protein contents of the phosphorylated forms of Akt (Ser473 and Thr308), K and S6 in HL-1 cells mechanically stretched for the indicated time. Cells were serum-starved for 4 h then incubated in DMEM supplemented with 0.1 % bovine fetal serum during cyclic stretch. n = 6. Means not sharing a common superscript are significantly different from each other, P < 0.05. Representative blots below graphs
