Figure 4.

Visualization of chlamydial IncA and OmcB expression by IFA in the presence or absence of Andro. (a) Representative C. trachomatis-infected cells co-stained with IncA (red) and GFP/MOMP (green). (b) Representative C. trachomatis-infected cells co-stained with OmcB (red) and GFP/MOMP (green). HeLa 229 cells infected with C. trachomatis L2GFP exposed to Andro (30 μM) for 44 h were fixed and processed for IFA. The IncA and OmcB proteins (red) were probed with polyclonal antibodies against IncA and OmcB, respectively. Chlamydial organisms were visualized by GFP and MOMP staining with anti-MOMP antibody (green). DNA was counterstained with DAPI (blue). Images were obtained under the same exposure conditions. Note: more abundant, scattered, OmcB-expressing EBs were identified in unexposed cells, but not in Andro-exposed cells as indicated by arrows. Scale bar = 10 μm.