| Firefly luciferase buffer reagents | Final concentration (1x) |
| Glycylglycine | 25 mM |
| KxPO4 (pH 7.8) | 15 mM |
| MgSO4 | 15 mM |
| DTT (store at 4º) | 1 mM |
| EGTA | 4 mM |
| ATP* | 2 mM |
| Beetle luciferin* | 250 μM |
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