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. 2015 Aug 20;22(8):1008–1017. doi: 10.1016/j.chembiol.2015.06.024

Figure 2.

Figure 2

In-Gel Fluorescent Analysis of Protein Fatty Acylation During HSV Infection in RPE-1 Cells

Cells were pulsed with YnPal or YnMyr (25 μM) for 6 hr and harvested at various times as shown. Proteins were isolated, processed for coupling to capture reagent AzTB, and analyzed for palmitoylation (A) or myristoylation (B). Arrows point to bands of increased fluorescence intensities (proteins with increased acylation levels) and arrowheads point to bands with decreased fluorescence intensities (proteins with decreased acylation levels).