Figure 1.

Protease-cleavable biosensors are activated by recombinant human granzymes. Luciferase was expressed by in vitro translation, and activation was tested after cleavage with recombinant human granzyme B (A-C), granzyme A (D), or granzyme K (E) over different concentrations. Recombinant granzyme A or K (0.42 U/uL) was preincubated with nafamostat mesylate before addition to GLS.SGR (granzyme A, K) or GLS.PGPR (granzyme A) (F). After 1 hour of incubation of in vitro translated luciferase with enzyme, luciferase reagent was added, RLU were measured, and fold activation or percentage inhibition was calculated as described in Methods. Error bars indicate standard error of the mean (SEM) for 3 experiments.