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. Author manuscript; available in PMC: 2015 Aug 21.
Published in final edited form as: J Trauma Acute Care Surg. 2014 Apr;76(4):982–990. doi: 10.1097/TA.0000000000000163

Figure 5.

Figure 5

Effect on BAL cellular composition with macrophage-depleting agent clodronate in the evolution of LC injury. A, An additional group of animals were sacrificed 48 hours after pretreatment injection; values show the composition of BAL before LC. B, Evolution of the total number of cells present in BAL after LC. Clodronate treatment decreased the total number of cells present in BAL at all compared time points. C, With the use of F4/80+/CD11b+ antibody labeling, macrophage numbers were measured by flow cytometry. Decreases in total number of cells were attributed overall to diminished number of total macrophages after clodronate treatment. D, Neutrophils measured by flow cytometry using Gr-1+/CD11b+/F4/80− labeling showed that within the clodronate group, there was a significant, more than 100-fold increase of neutrophilic at 5 hours. Neutrophilia was persistent at all time points. One-way ANOVA versus uninjured control (n = 9, *p < 0.05. **p < 0.01. ***p < 0.001). One-way ANOVA versus liposome (n = 9, ##p < 0.01. ###p < 0.001).