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. Author manuscript; available in PMC: 2015 Aug 21.
Published in final edited form as: Neuron. 2013 Sep 18;79(6):1109–1122. doi: 10.1016/j.neuron.2013.08.003

Figure 1. Loss of Tet1 does not affect brain size and morphology but leads to a reduction in 5hmC levels in the cortex and hippocampus.

Figure 1

(A,B) Global genomic 5hmC levels are significantly reduced, and 5mC levels are slightly but not significantly increased, in the cortex and hippocampus of Tet1KO mice compared to control Tet1+/+ animals. Graphic representation of 5hmC and 5mC levels in the cortex and hippocampus of Tet1+/+ (black) and Tet1KO (red) mice analyzed by liquid chromatography/mass spectrometry multiple reaction monitoring (LC/MS/MS-MRM) (3 + 3 animals). (ns - not significant; *P<0.05; error bars +/− stdev).

(A) Representative images of the cingulate cortex and hippocampus CA1 are shown. (red – 5hmC; green – NeuN).

(B) Graphs showing the global genomic levels of 5mC and 5hmC in the cortex and hippocampus of Tet1+/+ (black) and Tet1KO (red) mice.

(C-E) Tet1KO mice have normal neuronal number in different brain areas (C,D) and normal total brain weight (E). (blue – Hoechst; green – NeuN; 3 + 3animals; scale bar – 100 μm; ns - not significant; *P<0.05; **P<0.01; error bars +/− sem). See also Figure S1.