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. Author manuscript; available in PMC: 2015 Aug 21.
Published in final edited form as: Neuron. 2013 Sep 18;79(6):1109–1122. doi: 10.1016/j.neuron.2013.08.003

Figure 3. Normal basal synaptic transmission and LTP but abnormally enhanced hippocampal LTD in Tet1KO mice.

Figure 3

(A,B) Tet1 mice demonstrate normal basal synaptic transmission. CA1 fEPSPs evoked by Schaffer collateral pathway stimulation in acute hippocampal slices.

(A) The input-output curve was obtained by plotting the slopes of fEPSP against fiber valley amplitudes.

(B) PPF ratio was plotted against various interstimulus intervals.

(C) Similar intact presynaptic excitability of hippocampal neurons in Tet1KO mice (red) compared to controls (black). Presynaptic fiber volley amplitude was plotted against various stimulus intensities (3 + 3 animals; 5 and 6 slices).

(D) Normal hippocampal LTP in Tet1KO mice. LTP in CA1 in acute hippocampal slices from 6-week-old Tet1+/+ (black) (4 animals, 7 slices) and littermate Tet1KO (red) (4 animals, 6 slices) mice evoked by 2 x theta-burst stimulation (TBS). fEPSP slope is shown as percentage of baseline. A bar graph shows the average of fEPSP as a percentage of baseline during the last 10 min of recording.

(E) Tet1KO mice exhibit abnormally increased hippocampal LTD. LTD was induced in the Shaffer collateral-CA1 synapses by single-pulse low frequency stimulation (900 stimuli, 1 Hz) in hippocampal slices from 4 pairs of behaviorally naive 6-week-old Tet1+/+ (black) and Tet1KO (red) littermate mice (8 slices per group). A bar graph represents the average of fEPSP as a percentage of baseline during the last 10 min of recording (ns - not significant; *P<0.05; **P<0.01; error bars +/− sem.). See also Figure S3.