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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: DNA Repair (Amst). 2015 Jun 17;33:24–34. doi: 10.1016/j.dnarep.2015.06.004

Figure 4. FEN1 flap cleavage during pol β bypass of a cdA.

Figure 4

FEN1 flap cleavage on the substrates containing a 5′-phosphate or 5′-THF residue with a 1-nt gap opposite a template 5′R-cdA or 5′S-cdA was examined as described in Materials and Methods. Lanes 1, 4, 7 and 10 represent substrates only. Lanes 2, 5, 8 and 11 correspond to reaction mixtures with 10 nM FEN1. Lanes 3, 6, 9 and 12 correspond to reaction mixtures with 10 nM FEN1 in the presence of 5 nM pol β. Substrates were 32P-labeled at the 3′-end of the downstream primer as indicated. Substrates are illustrated schematically above the gel.