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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Mol Cancer Res. 2015 May 20;13(8):1238–1247. doi: 10.1158/1541-7786.MCR-14-0674-T

Figure 4. Knockdown of CUL3 causes increased growth in lung cancer cell lines.

Figure 4

CUL3 knock down in H522 cells increases cell proliferation and anchorage-independent cell growth. A) Western blot indicating that CUL3 is knocked down in H522 cells. B) Cell proliferation assay showing that CUL3 knockdown cells proliferate more rapidly than the non-silencing negative control (t-test day 5 p-value < 0.05). C) Images of colonies growing in soft agar in Non-silencing (left panel) and CUL3 knockdown (right panel) H522 cells. D) Quantification of 12 replicates of the soft agar assay indicating that CUL3 knockdown leads to increased colony formation in soft agar (t-test p-value < 0.05).