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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Mol Cancer Res. 2015 May 20;13(8):1238–1247. doi: 10.1158/1541-7786.MCR-14-0674-T

Figure 5. Double knockdown of CUL3 and PTEN results in increased growth.

Figure 5

Knockdown of CUL3 and PTEN in A549 cells increases cell proliferation and anchorage-independent growth. A) Western blot showing knockdown of PTEN, CUL3, or PTEN and CUL3 in A549 cells. B) Soft Agar Assay shows that knocking down both PTEN and CUL3 in A549 cells leads to increased colony formation compared to controls. In addition, the single CUL3 knockdown formed more colonies than the PTEN knockdown (t-test p-value ** ≤ .01, p-value *** ≤ .001). C) MTS based cell proliferation assay indicates that knocking down both CUL3 and PTEN leads to increased cell growth compared to knocking down CUL3 or PTEN or the negative control. CUL3 knock down also led to increased growth compared to knocking down PTEN or the negative control (t-test p-values day 5 < 0.01, all comparisons).