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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Mol Cancer Res. 2015 May 11;13(8):1197–1205. doi: 10.1158/1541-7786.MCR-15-0089

Figure 2. DNA damage signaling is inhibited by Nutlin and requires Mdm2.

Figure 2

(A, C) p53−/− MEFs and (B, D) p53−/−Mdm2−/− MEFs were treated with Nutlin (10 µM) or vehicle control (DMSO). MEFs with ectopic Mdm2 overexpression served as a positive control. Following exposure to 5 Gy of γ-radiation, MEFs were fixed at intervals and immunofluorescence for γH2AX (A, B) and pS/TQ (C, D) was performed. The number of foci per cell was quantified. The mean of 2–3 independent experiments is graphed. Error bars represent SEM, and significance (*) determined using a confidence interval of 95%.