Fig 10. Expression of pro-inflammatory cytokines/chemokines in the CNS compartments and muscle tissue of EV71 (C4)-infected mice.

The vaccinated Tg mice were subcutaneously infected with 1×10⁶ pfu of the EV71 3340 (C4) strain, and the RNAs extracted from the brain stem, spinal cord, and muscle at 6 days post-infection were subjected to quantitative RT-PCR analysis. Mock (M): uninfected mice group; Virus (V): EV71 3340 (C4) infected mice group. A schematic representation of the target gene expression and the statistical average from 5 mice per group is shown. The used vaccine formulations are as labeled. Significant difference between each group was shown as *: p ﹤ 0.05, **: p ﹤ 0.01, and ***: p ﹤ 0.001.