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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Mol Cancer Res. 2015 May 20;13(8):1206–1217. doi: 10.1158/1541-7786.MCR-15-0169

Figure 3.

Figure 3

ZBTB7A expression negatively correlates with MCAM expression in both human melanoma patient samples and melanoma cell lines. A, the levels of ZBTB7A mRNA and MCAM mRNA in different stages of human melanoma were analyzed based on the values derived from Kabbarah dataset. Error bars represent mean ± s.e.m. B, the correlation between ZBTB7A and MCAM mRNA expression derived from two different human melanoma datasets was shown. C, the mRNA levels of 12 human melanoma cell lines were assessed by real time PCR, 18s served as internal control for the analysis. Average values of three independent experiments are shown as mean ± s.e.m. D and E, the correlation of ZBTB7A mRNA with MCAM mRNA in 12 human melanoma cell lines was analyzed. ZBTB7A low expression cell lines (A375, SK-Mel-2, UACC62 and UACC257) express an average 12-fold more MCAM than ZBTB7A high expression cell lines (1205Lu, Lox-IM VI, MeWo and WM155). F, ZBTB7A and MCAM protein expression detected by Western blot in 12 different human melanoma cell lines, β-actin served as loading control.