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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Biochem Pharmacol. 2015 Jul 23;97(1):77–88. doi: 10.1016/j.bcp.2015.06.036

Fig. 2.

Fig. 2

Effects of hypoxia/reoxygenation (H/R) on smooth muscle contractility and AMPK phosphorylation in rat aortic smooth muscle ex vivo. Endothelium-denuded rat aortic rings were maintained under normoxic or H/R conditions as described under ‘Materials and methods’. A–B) The changes in Emax and pEC50 values for 5-HT-induced contractions were determined. The data shown are the mean ± SEM values obtained with aortic rings from 3 rats. ##p < 0.01, ###p < 0.001 compared with control (+ 5-HT, Norm); **p < 0.01 compared with 0 mM D-glucose (+ 5-HT, H/R). The changes in AMPKαThr172 phosphorylation were then determined. The representative immunoblots for phospho-AMPKαThr172, AMPKα, and β-actin are shown in panel C.