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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Prog Retin Eye Res. 2015 Apr 17;48:181–202. doi: 10.1016/j.preteyeres.2015.04.001

Figure 7.

Figure 7

Fixative type gives a differential staining pattern of HCN4 antibody in rat retina. Whole-mounted retinae (from opposite eyes of same animal) fixed in (A) 4% formaldehyde or (B) modified Davidson’s fluid, processed in parallel, and stained with anti-HCN4 primary antibody and Alexa Fluor 488-conjugated anti-mouse secondary antibody (green). Each panel shows a single optical section through the ganglion cell layer, collected on a laser scanning confocal microscope. Scale bar in (B) is 10 µm and applies to both panels.