Figure 4.

Nobiletin limits thrombus formation in vitro and extends bleeding time in mice. DiOC₆ labelled human blood was pre-incubated with vehicle or different concentrations of nobiletin and perfused over collagen-coated Vena8 BioChips. Images were obtained every 30 s for up to 10 min and representative images (at 0, 2, 4, 6, 8 and 10 min) are shown in (A). The fluorescence intensity of thrombi obtained in the absence of nobiletin was taken as 100% and compared with the levels obtained with different concentrations of nobiletin (B). Data represent mean ± SD (n = 4). The P-values shown in the figure are as calculated by non-parametric repeated measures anova (Friedman test; ****P < 0.0001). The effect of nobiletin (estimated 50 μM) on haemostasis in mice was analysed by measuring the bleeding time after removal of 1 mm of tail tip. The bleeding time obtained with vehicle-treated group was compared with nobiletin-treated mice (C). Data represent mean ± SD (n = 7 mice for vehicle control or nobiletin-treated group). The significance between control and treated groups, and the P-values were calculated by Student's t-test.