Figure 5.

Nobiletin inhibits phosphorylation of PLCγ2 and PKB (Akt). Washed human platelets were stimulated with CRP-XL (1 μg·mL⁻¹) in the absence or presence of various concentrations of nobiletin before the analysis by immunoblotting using anti-phosphotyrosine antibody (A) and phospho-specific antibodies for proteins involved in the GPVI pathway such as Syk pY323, LAT pY200, PLCγ2 pY759 and Akt pS473 (B). Total level of 14-3-3ζ was measured on each sample as a loading control. The blots shown in the figure are representative of four separate experiments. R represents resting platelets. Data presented in (B) represent mean ± SD (n = 4) and P-values were calculated using non-parametric repeated measures anova (Friedman test; ****P < 0.0001).