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. 2015 Jul 8;290(34):20687–20699. doi: 10.1074/jbc.M115.654863

FIGURE 7.

FIGURE 7.

The effects of NR4A1 knockdown on the expression of Survivin and Bcl-2 and on ER stress-induced apoptosis in MIN6 cells. Analyses of MIN6 cell stably knockdown NR4A1 clone (designated as KD) and normal control clone (designated as CON). A, protein expression of NR4A1, Bcl-2, and Survivin in CON and KD cells. B and C, the cell viability of CON and KD cells treated with TG (B) or PA (C) for 24 h was analyzed with an MTT assay. D, changes in protein expression of NR4A1, phosphorylated eif2α (P-eif2α), CHOP, and Caspase3 (17 kDa) in CON and KD cells treated with 0.5 μm TG. Densitometric analyses of the Western blots are shown as histograms or curves. The data show the means of three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus CON.