FIGURE 3.

Mapping the interaction domains of MCPIP1 and MCPIP4. A, scheme for generation of the expression constructs encoding the serial deletions of MCPIP1 and MCPIP4 as indicated. B and C, mammalian two-hybrid assay for the interaction of MCPIP1 and MCPIP4. Different combinations of pBIND- and pACT-derived expression vectors were co-transfected with a reporter containing five Gal4 binding sites upstream of a minimum promoter-drive luciferase gene into HEK293 cells. The luciferase activity was measured using a dual-luciferase assay system. Data are presented as mean ± S.D., n = 4.