FIGURE 7.

The CTD is dispensable for NEIL1 base excision/AP lyase activity but is required for optimum repair activity involving replication proteins. A, complete LP-BER assay with pUC19CPD as in Fig. 4D, using recombinant proteins, shows proficient repair with WT NEIL1 but not with the nested CTD deletion polypeptides aa 1–349 and 1–311. The 1–288 mutant was inactive, as shown previously (30). B, similar results were obtained with linear duplex oligonucleotide substrate for WT NEIL1 and N311 mutant. C, base excision/AP lyase activity was comparable for WT NEIL1, 1–349, and 1–311. The 1–288 polypeptide was inactive. Error bars, S.E.