FIGURE 1.

RCAN1.4 protein expression is induced by PACAP. A, use of different promoters to produce RCAN1 isoforms. B and C, PC12 cells were treated with PACAP (10 nm) for the indicated times. The cell extracts were immunoblotted with anti-RCAN1 and anti-GAPDH antibodies (B) and then quantified (C). D and E, PC12 cells were treated with the indicated concentrations for 3 h. The cell extracts were immunoblotted with anti-RCAN1 and anti-GAPDH antibodies (D) and then quantified (E). F and G, mouse primary cortical neurons were treated with PACAP (10 nm) for the indicated times. The cell extracts were immunoblotted with anti-RCAN1 and anti-GAPDH antibodies (F) and then quantified (G). Data are mean ± S.D. of three independent experiments. **, p < 0.01.