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FHA-mediated dimerization is important for the ability of Mdb1 to form nuclear foci. A, focus formation by Mdb1 requires FHA-mediated dimerization. Cells were imaged before and 30 min after exposure to 36 Gy of IR. The strains used were DY15603, DY15638, DY16309, and DY16307. Bar, 5 μm. B, a time course analysis of IR-induced Mdb1 focus formation. Approximately 200 nuclei were examined for each sample.
